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KMID : 0861020210360020031
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Korea Journal of Herbology
2021 Volume.36 No. 2 p.31 ~ p.42
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Protective Effect of Spatholobi Caulis in Thioacetamide induced Acute Liver Injury of Rat
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Oh Min-Hyuck
Shin Mi-Rae
Roh Seong-Soo
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Abstract
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Objectives : This study was undertaken to investigate the hepatoprotective effect of Spatholobi Caulis water extract (SC) to thioacetamide (TAA)-induced acute liver injury (ALI) in rats.
Methods : The rats were injected intraperitoneally with TAA (200 ㎎/㎏ body weight) and orally administered SC (100 or 200 ㎎/㎏ b.w.) daily for 3 days. Liver biomarkers were assessed by serum glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and ammonia levels. Malondialdehyde (MDA) was measured both serum and liver tissue. In addition, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, anti-oxidant, and inflammation-related proteins were investigated by western blot analysis. Histological examination further confirmed though hematoxylin and eosin stain.
Results : The SC treatment reduced liver function markers like GOT and GPT and also remarkably decreased ammonia level. Moreover, the elevated MDA level in TAA-induced group was significantly reduced by SC treatment. NADPH oxidase expression associated with oxidative stress including NOX2, NOX4, and p47phox markedly inhibited by SC administration. SC treatment exerted anti-oxidant effect through the increase of anti-oxidant enzyme including superoxide dismutase (SOD), Catalase, and heme oxygenase-1 (HO-1). The protein expressions of inflammatory cytokines such as tumor necrosis factor-α (TNF-α), IL-6, and IL-1β induced by nuclear factor-kappa B (NF-κB) activation were modulated through blocking the phosphorylation of inhibitor of nuclear factor κBα (IκB)α. SC treatment also improved histological alterations.
Conclusion : These findings suggested that SC administration may be a potential candidate for the prevention or treatment of ALI.
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KEYWORD
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Acute liver injury, Spatholobi Caulis water extract, Thioacetamide, Oxidative stress, Anti-oxidant, Anti-inflammation
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